(2S)-2-Amino-4-(butylsulfonimidoyl)butanoic acid (BD136012) is a building block containing a sulfoximine group. Several CDK and ATR inhibitors have exemplified the utilization of the NH sulfoximine group as abioisostere for a sulfonamide group to overcome the main project hurdles of aqueous solubility, sulfonamide-mediated off-target activity and IP. Moreover, its NH group could be expediently further functionalized through Buchwald-Hartwig coupling reaction and multifarious nucleophilic reactions.. Recommended Products is: 4381-25-3 and 1621962-30-8.
Our previous study revealed that N-acetyl-L-cysteine (NAC) could enhance the secretion of recombinant proteins by Pichia pastoris, but the corresponding mol. mechanisms are still unclear. In the present study, we explored whether other thiols have a similar action on the secretion of recombinant human serum albumin and porcine FSH fusion protein (HSA-pFSH¦Â), to reveal the mechanism of NAC on HSA-pFSH¦Â secretion. Transcriptome anal. showed that genes involved in oxidoreductase activity and oxidation-reduction process were upregulated in cells supplemented with NAC. The other three thiol-reducing regents including dimercaptopropanol (DT), thioglycolic acid, and mercaptolactic acid could improve HSA-pFSH¦Â production in the culture supernatant. Among them, only DT had similar effect as NAC on HSA-pFSH¦Â secretion and the increase of GSH content. Moreover, 1-20 mM GSH, 1-10 mM cysteine, or 1-20 mM N-acetyl-D-cysteine supplementation could improve the secretion of HSA-pFSH¦Â. Furthermore, 0.4-3.2 mM ethacrynic acid, rather than 1-16 mM BSO could inhibit the effect of NAC on the production of HSA-pFSH¦Â. These results indicated that NAC improved the secretion of HSA-pFSH¦Â by increasing the intracellular GSH content through its thiol activity rather than as a precursor for GSH synthesis. In conclusion, our results demonstrate, for the first time, that the secretion of recombinant HSA-pFSH¦Â in Pichia pastoris could be improved through thiol-reducing agent supplementation, and the mechanism of the effect NAC has on HSA-pFSH¦Â secretion is associated with improving the intracellular GSH content.
The mechanism of N-acetyl-L-cysteine in improving the secretion of porcine follicle-stimulating hormone in Pichia pastoris. Recommended basis is Sulfoximine, Bioisosteric. Products is: https://www.ambeed.com/products/50578-18-2.html, 145026-07-9
Referemce:
Benzoxazole – Wikipedia,
Benzoxazole | C7H5NO – PubChem