Synthetic Route of 2008-04-0, The reaction rate of a catalyzed reaction is faster than the reaction rate of the uncatalyzed reaction at the same temperature.2008-04-0, Name is 2-(Trifluoromethyl)benzo[d]oxazole, molecular formula is C8H4F3NO. In a Article£¬once mentioned of 2008-04-0
Nitrile-synthesizing enzyme: Screening, purification and characterization
Cyanide is known as a toxic compound for almost all living organisms. We have searched for cyanideresistant bacteria from the soil and stock culture collection of our laboratory, and have found the existence of a lot of microorganisms grown on culture media containing 10 mM potassium cyanide. Almost all of these cyanide-resistant bacteria were found to show beta-cyano-L-alanine (beta-CNAla) synthetic activity. beta-CNAla synthase is known to catalyze nitrile synthesis: the formation of beta -CNAla from potassium cyanide and O-acetyl-L-serine or L-cysteine. We found that some microorganisms were able to detoxify cyanide using O-methyl-DL-serine, O-phospho-L-serine and beta-chloro-DL-alanine. In addition, we purified beta-CNAla synthase from Pseudomonas ovalis No. 111 in nine steps, and characterized the purified enzyme. This enzyme has a molecular mass of 60,000 and appears to consist of two identical subunits. The purified enzyme exhibits a maximum activity at pH 8.5?9.0 at an optimal temperature of 40?50oC. The enzyme is specific for O-acetyl-L-serine and beta-chloro-DL-alanine. The Km value for O-acetyl-L-serine is 10.0 mM and Vmax value is 3.57 mumol/min/mg.
A reaction mechanism is the microscopic path by which reactants are transformed into products. Each step is an elementary reaction. In my other articles, you can also check out more blogs about 2008-04-0
Reference£º
Benzoxazole – Wikipedia,
Benzoxazole | C7H5NO – PubChem