Research speed reading in 2021. Chemistry is a science major with cience and engineering. The main research directions are chemical synthesis, new energy materials, preparation and modification of special coatings. 409071-16-5, Name is Lithium difluoro(oxalato)borate, molecular formula is , belongs to benzoxazole compound. In a document, author is de Paiva, Fernanda C. R., Recommanded Product: 409071-16-5.
Adenylate-forming enzymes (AFEs) are a mechanistic superfamily of proteins that are involved in many cellular roles. In the biosynthesis of benzoxazole antibiotics, an AFE has been reported to play a key role in the condensation of cyclic molecules. In the biosynthetic gene cluster for the benzoxazole AJI9561, AjiA1 catalyzes the condensation of two 3-hydroxyanthranilic acid (3-HAA) molecules using ATP as a co-substrate. Here, the enzymatic activity of AjiA1 is reported together with a structural analysis of its apo form. The structure of AjiA1 was solved at 2.0 angstrom resolution and shows a conserved fold with other AFE family members. AjiA1 exhibits activity in the presence of 3-HAA (K-m = 77.86 +/- 28.36, k(cat) = 0.04 +/- 0.004) and also with the alternative substrate 3-hydroxybenzoic acid (3-HBA; K-m = 22.12 +/- 31.35, k(cat) = 0.08 +/- 0.005). The structure of AjiA1 in the apo form also reveals crucial conformational changes that occur during the catalytic cycle of this enzyme which have not been described for any other AFE member. Consequently, the results shown here provide insights into this protein family and a new subgroup is proposed for enzymes that are involved in benzoxazole-ring formation.
Balanced chemical reaction does not necessarily reveal either the individual elementary reactions by which a reaction occurs or its rate law. In my other articles, you can also check out more blogs about 409071-16-5. Recommanded Product: 409071-16-5.
Reference:
Benzoxazole – Wikipedia,
,Benzoxazole | C7H5NO – PubChem