Reference of 2008-04-0, Because a catalyst decreases the height of the energy barrier, its presence increases the reaction rates of both the forward and the reverse reactions by the same amount.2008-04-0, Name is 2-(Trifluoromethyl)benzo[d]oxazole, molecular formula is C8H4F3NO. In a article£¬once mentioned of 2008-04-0
A new bacterial L-amino acid oxidase with a broad substrate specificity: Purification and characterization
The Gram-positive bacterium Rhodococcus opacus DSM 43250 produces an L-amino acid oxidase (L-AAO) with a very broad substrate specificity. This enzyme has been purified to homogeneity and a detailed biochemical characterization was carried out. The complete nucleotide sequence of the L-AAO gene was determined and the primary structure of L-AAO was deduced. The molecular mass of the native enzyme was 99kDa determined by gel filtration, 54.2/108.5kDa measured by MALDI-TOF/MS, 53.2kDa for the subunit calculated after SDS/PAGE. The coenzyme-binding motif G-X-G-X-X-G which is known for all L-AAOs was found very close to the N-terminus of the protein. L-AAO oxidized 39 out of 43 tested L-amino acids. The kinetic data for 16 of these L-amino acids were determined revealing Km-values in the range of 15-30muM for substrates like L-phenylalanine, L-leucine, L-citrulline and L-lysine. The stability of L-AAO can be increased by storage or incubation of the enzyme in glycine/NaOH buffer. The protein has a pI of 4.8 and a slightly basic pH-optimum at pH 8-9 measured for L-alanine, L-phenylalanine and L-leucine as substrates. The ability for resolution of racemic mixtures was investigated and D-amino acids with an enantiomeric excess of >99% were obtained.
A reaction mechanism is the microscopic path by which reactants are transformed into products. Each step is an elementary reaction. In my other articles, you can also check out more blogs about 2008-04-0
Reference£º
Benzoxazole – Wikipedia,
Benzoxazole | C7H5NO – PubChem